网站首页    代理品牌    PsaL / Anti-PsaL subunit of Photosystem I antibody PHY0064

PsaL / Anti-PsaL subunit of Photosystem I antibody PHY0064

PsaL / Anti-PsaL subunit of Photosystem I antibody
Catalog Number: PHY0064
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT4G12800

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin to ferredoxin. PsaL is binding pigments and has been shown to be involved in trimerization of PSI in cyanobacteria (but not in plants) and bind pigments in plants and cyanobacteria.

  Synonyms:

PsaL, PSI-L

  Immunogen:

KLH-conjugated synthetic peptide of PsaL derived from Arabidopsis thaliana AT4G12800.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

18 / 15 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Brassica rapa, Zea mays, and 80-99% homologues with the sequence in Populus trichocarpa, Gossypium raimondii, Medicago truncatula, Solanum tuberosum, Solanum lycopersicum, Populus trichocarpa, Vitis vinifera, Cucumis sativus, Oryza sativa Japonica Group. 
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1 μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.