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AtpD / Anti-Delta subunit of chloroplast ATP synthase antibody PHY0314

AtpD / Anti-Delta subunit of chloroplast ATP synthase antibody
Catalog Number: PHY0314
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT4G09650

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

ATP synthase subunit delta is part of the stalk that links CF0 to CF1. It either transmits conformational changes from CF0 to CF1 or is implicated in proton conduction.

  Synonyms:

AtpD, F-type H+-transporting ATPase subunit delta, ATP synthase F(1) sector subunit delta, F-type ATPase subunit delta

  Immunogen:

Recombinant full length delta subunit of chloroplast ATP synthase of Arabidopsis thaliana AT4G09650.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:5000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

25.7 / 20 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

1-3 is thylakiod membrane protein from Arabidopsis thaliana leaf containing 0.1μg, 0.25μg, and 0.5μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.