The CFoIII subunits of chloroplast ATP synthase (AtpH) is an 8 kD integral membrane protein with two transmembrane domains.
AtpH, ATP synthase F(0) sector subunit c, Lipid-binding protein Dicyclohexylcarbodiimide-binding protein, F-type ATPase subunit c, F-ATPase subunit c
KLH-conjugated synthetic peptide of AtpH derived fromArabidopsis thalianaATCG00140.
Immunogen affinity purified
Reconstitution with 150µl of 0.01 M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence inMedicago truncatula, Oryza sativa Japonica Group, Glycine max, Nicotiana tabacum,and 80-99% homologues with the sequence inPhyscomitrella patens, Chlamydomonas reinhardtii. For more species homologues information, please contact tech support at email@example.com.
1-3 is thylakoid membrane protein fromArabidopsis thalianaleaf containing 0.25μg, 0.5μg, and 1μg of chlorophyll, respectively. Electrophoresis:Tricine-SDS-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:1000 dilution overnight at 4℃. Secondary antibody:1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection: using chemiluminescence substrate and image were captured with CCD camera.