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AtpH / Anti-CFoIII subunits of chloroplast ATP synthase antibody PHY0114A

AtpH / Anti-CFoIII subunits of chloroplast ATP synthase antibody
Catalog Number: PHY0114A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: ATCG00140

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

The CFoIII subunits of chloroplast ATP synthase (AtpH) is an 8 kD integral membrane protein with two transmembrane domains.

  Synonyms:

AtpH, ATP synthase F(0) sector subunit c, Lipid-binding protein Dicyclohexylcarbodiimide-binding protein, F-type ATPase subunit c, F-ATPase subunit c

  Immunogen:

KLH-conjugated synthetic peptide of AtpH derived from Arabidopsis thaliana ATCG00140.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

8 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Medicago truncatula, Oryza sativa Japonica Group, Glycine max, Nicotiana tabacum, and 80-99% homologues with the sequence in Physcomitrella patens, Chlamydomonas reinhardtii.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

 Example 1

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.25μg, 0.5μg, and 1μg of chlorophyll, respectively. 
Electrophoresis: Tricine-SDS-PAGE 
Transfer: blotting to NC (nitrocellulose) membrane for 1h. 
Blocking: 5% skim milk at RT or 4℃ for 1h. 
Primary antibody: 1:1000 dilution overnight at 4℃. 
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) 
Detection: using chemiluminescence substrate and image were captured with CCD camera.