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Sec22 / Anti-25.3 kDa vesicle transport protein antibody AT1G11890

Catalog Number: PHY0943S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT1G11890

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

AtSec22 is a member of SEC22 Gene Family. AtSec22 is involved in vesicle trafficking from the ER to the Golgi complex.

  Synonyms:

Sec22, AtSec22, SEC22, SECRETION 22.

  Immunogen:

KLH-conjugated synthetic peptide of AtSec22 derived from Arabidopsis thaliana AT1G11890.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000), Immunoblotting 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

25 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity:  

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Zea mays, Sorghum bicolor.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 20 μg total protein from Arabidopsis thaliana leaf. 
Lane 2: 40 μg total protein from Arabidopsis thaliana leaf. 
Lane 3: 80 μg total protein from Arabidopsis thaliana leaf. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.