AtSec22 is a member of SEC22 Gene Family. AtSec22 is involved in vesicle trafficking from the ER to the Golgi complex.
Sec22, AtSec22, SEC22, SECRETION 22.
KLH-conjugated synthetic peptide of AtSec22 derived fromArabidopsis thalianaAT1G11890.
Serum Peptide affinity form antibody available upon request at firstname.lastname@example.org.
Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000), Immunoblotting Note: Optimal dilutions/concentrations should be determined by the end user.
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence inZea mays, Sorghum bicolor. For more species homologues information, please contact tech support at email@example.com.
Lane 1: 20 μg total protein fromArabidopsis thalianaleaf. Lane 2: 40 μg total protein fromArabidopsis thalianaleaf. Lane 3: 80 μg total protein fromArabidopsis thalianaleaf. Electrophoresis:15% SDS-Urea-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:1000 dilution overnight at 4℃. Secondary antibody:1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.