PDE2A Assay Kit 60320-代理进口生物技术-启维益成

Background:

Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE2A, also known as cGMP-stimulated phosphodiesterase, hydrolyzes cyclic nucleotides cAMP (Km = 2.4 µM) and cGMP, and is involved in the regulation of blood pressure and fluid homeostasis. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.

Description:

The PDE2A Assay Kit is designed for identification of PDE2A1 inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE2A1 to the binding agent. The key to the PDE2A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE2A1 reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE2A1 for 1 hour. Second, binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence readerequipped for the measurement of fluorescence polarization.

Synonym(s):

inhibitor screening, assay kit, PDE2A

Supplied As:

The PDE2A inhibitor screening assay kit comes in a convenient 96-well format, with purified PDE2A1 enzyme, fluorescently labeled PDE2A1 substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions.

Contraindications:

DMSO >1%, strong acids or bases, ionic detergents, high salt

Format:

Assay Kit Components

Instructions for use:

See assay kit data sheet for detailed protocol.

Storage / Stability:

At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Reference(s):

Maurice DH. Front. Biosci. 2005; 10:1221-8.

Notes:

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable to measure fluorescence polarization

Warning(s):

Avoid freeze/thaw cycles

Scientific Category:

PDE

Product Type:

Assay Kit