BPSbioscience-11

40530 eIF4E, GST-tag

Catalog #40530
Size: 100 µg
Description
Human eIF4e (eukaryotic translation initiation factor 4E) (GenBank Accession No. NM_001968), a.a. 2-216 with N-terminal GST-tag, MW= 51 kDa, expressed in an E. coli expression system.
Species
Human
 
Host Species
E. coli
 
MW
51 kDa
 
Genbank #
NM_001968
 
Tag(s)
N-terminal GST-tag
 
a.a.
2–216
 
UniProt #
P06730
 
Applications
Suitable substrate for MNK1 kinase and other kinase assays.
 
Storage/Stability

>12 months at -80°C.

 
Shipping Temperature
-80
 
Synonym(s)
EIF4E1, Eukaryotic Translation Initiation Factor 4E, mRNA Cap-Binding Protein, EIF-4E
 
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
 
Format
Aqueous buffer solution
 
References

1. Shih,J.W. et al., Oncogene 27(5), 700-714 (2008). 
2. Salehi Z., Mashayekhi F. and Shahosseini F. Cell Biol. Int. 31(11), 1400-1404 (2007).

Application Reference: 
MNK1-induced eIF-4E phosphorylation in myeloma cells: a pathway mediating IL-6-induced expansion and expression of genes involved in metabolic and proteotoxic responses (2014)

 
Warnings
Avoid freeze/thaw cycles
 
Scientific Category
Kinase/Substrate