51056 SetDB1 Chemiluminescent Assay Kit-代理进口生物技术-启维益成

Description

The SetDB1 Chemiluminescent Assay Kit is designed to measure SetDB1 activity for screening and profiling applications. The key to the SetDB1 Direct Activity Assay Kit is a highly specific antibody that recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

UniProt #

Q15047

Applications

Useful for studying enzyme kinetics and HTS applications.

Supplied As

Kit comes in a convenient format, with wells precoated with histone H3 peptide substrate, antibody against methylated lysine residue of Histone H3, secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase buffer, and purified enzyme

Storage/Stability

One year from date of receipt when stored as directed.

Shipping Temperature

-80C

Synonym(s)

Histone-lysine Nmethyltransferase SETDB1, SET domain bifurcated 1, KMT1E, ERG-associated protein with SET domain (ESET)

Format

Instructions for Use

See assay kit data sheet for detailed protocol.

References

Dillon SC, Zhang X, Trievel RC, Cheng X. Genome Biology 2005; 6:227.

Notes

MATERIALS REQUIRED BUT NOT SUPPLIED:
TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Methyltransferases