Catalog #79560 RIPK1 Kinase Assay Kit-代理进口生物技术-启维益成

Materials Required But Not Supplied

ADP-Glo® Kinase Assay (Promega #V6930)

Dithiothreitol (DTT, 1 M; optional)

Microplate reader capable of reading luminescence

Adjustable micropipettor and sterile tips

30°C incubator

Applications

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Supplied As

The RIPK1 Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant RIPK1 enzyme, RIPK1 substrate, ATP and kinase assay buffer for 100 enzyme reactions.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Background

RIPK1 is a member of the receptor-interacting protein kinase (RIP) family. It plays an important role in cell survival as well as cell death including apoptosis and necroptosis. RIPK1 is highly expressed in Alzheimer’s disease (AD) brains. Importantly, it was shown that inhibition of RIPK1 results in decrease of amyloid and inflammation levels as well as accelerating amyloid beta peptide (Aβ) degradation by microglia in vitro.

Synonym(s)

RCK, MAP3K19, receptor-interacting serine/threonine-protein kinase 1 isoform 1, receptor-interacting serine/threonine-protein kinase 1, serine/threonine-protein kinase RIP, cell death protein RIP, receptor-interacting protein 1, receptor (TNFRSF)-interact

Format

Components

Instructions for Use

See assay kit data sheet for detailed protocol.

References

Ofengeim D., Proc. Natl. Acad. Sci. USA 114(41): E8788-E8797 (2017)

Warnings

Avoid freeze/thaw cycles.