SYBR Green I荧光染料(电泳)10000x
dsGreen Gel Staining Solution, 10000×
20010 1ml
20010 1ml
SYBR Green I is a sensitive dsDNA binding dye, which can be used for routine DNA detection in agarose and polyacrylamide gels.
Unlike Ethidium, SYBR Green I is highly selective towards double stranded DNA, much less harmful, and offer better sensitivity.
A comparison between Ethidium Bromide and SYBR Green I
Feature | Ethidium Bromide | SYBR Green I |
---|---|---|
Fluorescence | Red (615 nm) | Green (524 nm) |
Excitation maximum | 302 nm | 454 nm |
Excitation light source | UV only | Blue light or UV |
Sensitivity | 2 ng / band(dsDNA) 100 ng / band (RNA) |
0.08 ng / band (dsDNA) 1-2 ng / band (oligonucleotides) |
Health hazard | High | Low |
General properties
Appearance: | orange solution |
Quality control: | NMR 1H (95%) and 13C, TLC, functional testing |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
MSDS: | Download |
Spectral properties
Excitation maximum, nm: | 454 |
Extinction coefficient at excitation maximum, Lmol-1cm-1: | 73000 |
Emission maximum, nm: | 524 |
Fluorescence quantum yield: | 0.8 |
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