RedSafe核酸染色溶液 21141

RedSafe™ Nucleic Acid Staining Solution
21141 1ml

完美EB替代品，新型核酸染料RedSafe^TM 安全、无毒、灵敏性高

RedSafe^TMNucleic Acid Staining Solution(20,000x)

订购信息

名称	货号	规格	价格（元）
RedSafe^TMNucleic Acid Staining Solution(20,000x)	21141	1ml	750

产品描述

RedSafe^TMNucleic Acid Staining Solution(20,000x)是一种新型安全的核酸染料，在琼脂糖凝胶中检测核酸的过程中替代传统的EtBr染料，结合DAN或RNA后发射出绿色荧光，这种新型染料有两个荧光发射峰，一个集中在309nm处，另一个在419nm处。另外，在514nm处有可见发射光，结合DNA后荧光发射集中在537nm，RedSafe^TMNucleic Acid Staining Solution(20,000x)和一样灵敏。使用方法也相似，跟强诱变剂EtBr相比，在Ames测试中RedSafe^TM较少引起突变。另外，在小鼠骨髓嗜染红细胞和精母细胞染色体畸变试验中RedSafe^TM都表现出阴性结果。因此，在琼脂糖凝胶中使用RedSafe^TMNucleic Acid Staining Solution(20,000x)来替代EtBr检测核酸的存在是明智的选择。

优势

可用于检测双链DNA和单链RNA

可替代溴化乙锭（EB）染料

和EtBr一样灵敏甚至更强的灵敏性

无毒，无诱变性和致癌性

无有害废弃物

储存条件

室温下至少12个月稳定性不变，为维持其稳定性，最好保存于4℃，至少24个月稳定性不变。

应用

在琼脂糖凝胶电泳中可使DNA和RNA条带可视化

可分离出亚克隆的DNA片段，而不会像EtBr一样引起突变。

Description

RedSafe™ Nucleic Acid Staining Solution (20,000x) is a new and safe nucleic acid staining solution. It is an alternative to the traditional ethidium bromide(EtBr) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, onecaentered at 309 nm and another at 419 nm. In addition, it has one visible excitation at 514 nm. The fluorescence emission of RedSafe™ bound to DNA is centered at 537 nm. RedSafeTM Nucleic Acid Staining Solution (20,000x) is as sensitive as EtBr. The protocol is also similar with a protocol of EtBr. The Ames test proves lower mutation withRedSafe™ Nucleic Acid Staining solution compare with EtBr. In addition, RedSafe™ Nucleic Acid Staining Solution (20,000x) has a negative result in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermaryspermatocyte chromosomal aberration test.

Therefore, it is wise to choose RedSafe™ Nucleic acid Stainig Solution (20,000x) instead of EtBr for detecting nucleic acid in agarose gels.

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