叶绿体内膜转运蛋白TIC40抗体

Catalog Number: PHY1248S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT5G16620

Product Information

Description:	Rabbit polyclonal antibody
Background:	TIC40 is a protein in the chloroplast inner membrane translocon complex. It is involved in protein precursor import into chloroplasts and reinsertion of proteins from the chloroplast stroma into the inner membrane. Three components of the chloroplast protein translocon, Tic110(AT1G06950), Hsp93 (ClpC), and Tic40(AT5G16620), have been shown to be important for protein translocation across the inner envelope membrane into the stroma.
Synonyms:	TIC40, PDE120, PIGMENT DEFECTIVE EMBRYO 120, TRANSLOCON AT THE INNER ENVELOPE MEMBRANE OF CHLOROPLASTS 40
Immunogen:	Recombinant protein of TIC40 derived from Arabidopsis thaliana AT5G16620.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Serum
Reconstitution:	Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	49 / 43 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

Lane 1: 20 μg total protein from Arabidopsis thaliana leaf.
Lane 2: 10 μl total chloroplast protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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