RCA抗体

Catalog Number: PHY2144S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT2G39730

Product Information

Description:	Rabbit polyclonal antibody
Background:	Rubisco activase (RCA) is a key photosynthetic protein, it catalyses the activation of Rubisco and thus plays an important role in photosynthesis.
Synonyms:	RCA, RUBISCO ACTIVASE
Immunogen:	KLH-conjugated synthetic peptide derived from C-terminal section of RCA protein in Arabidopsis thaliana AT2G39730.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Serum Peptide affinity form antibody available upon request at info@phytoab.com.
Reconstitution:	Reconstitution with 150 µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	52 kDa / 42 kDa (α), 45 kDa (β) NOTE: In Arabidopsis, there are two forms of activase (alpha and beta), Alpha is about 46-47 kDa, beta is about 42 kDa.
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Solanum lycopersicum, Cucumis sativus, Populus trichocarpa, Medicago truncatula, Oryza sativa Japonica Group, Spinacia oleracea, Oryza sativa Indica Group. For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

Lane 1: 10 μl total chloroplast protein from Arabidopsis thaliana leaf .
Lane 2: 12 μg stromal protein from Arabidopsis thaliana leaf.
Lane 3: Thylakoid membrane protein from Arabidopsis thaliana leaf containing 5 μg of chlorophyll.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

首页 ꄲ 代理品牌 ꄲ RCA抗体

搜索