OLE1 / Anti-Oleosin 18.5 kDa antibody

Catalog Number: PHY0954A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT4G25140

Product Information

Description:	Rabbit polyclonal antibody
Background:	OLE1 is a protein found in oil bodies, and it is involved in seed lipid accumulation. Suppression of OLEO1 (and OLEO2 (AT5G40420)) resulted in an aberrant phenotype of embryo cells that contain unusually large oilbodies that are not normally observed in seeds.
Synonyms:	OLE1, OLEO1, OLEOSIN 1
Immunogen:	KLH-conjugated synthetic peptide of OLE1 derived from Arabidopsis thaliana AT4G25140.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Immunogen affinity purified
Reconstitution:	Reconstitution with 150µl of 0.01 M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:5000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	19 / 18 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica napus. For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

40 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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