RpoC1单克隆抗体

Catalog Number: PHY1904
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: ATCG00180

Product Information

Description:	Mouse monoclonal (Clone: 5B1) antibody
Background:	In chloroplasts, transcription of plastid genes is mediated by two types of RNA polymerase: plastid-encoded RNA polymerase (PEP) and nuclear encoded RNA polymerase (NEP). PEP is composed of four core subunits (α, β, β’, β’’) and a promoter recognition subunit (σfactor). RpoC1 (ATCG00180) is the β’subunit of PEP.
Synonyms:	RpoC1
Immunogen:	Recombinant protein of RpoC1(1-144aa) derived from Arabidopsis thaliana ATCG00180.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Protein A purified
Reconstitution:	Reconstitution with 150µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	79 / 72 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

Lane 1: 5 μg total protein from Arabidopsis thaliana leaf.
Lane 2: 10 μg total protein from Arabidopsis thaliana leaf.
Lane 3: 20 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:5000 dilution using Goat Anti-Mouse IgG H&L (HRP) (Cat# PHY6006)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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