ATP1 / Anti-ATP synthase subunit 1, mitochondrial antibody

Catalog Number: PHY2146S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: ATMG01190 AT2G07698

Product Information

Description:	Rabbit polyclonal antibody
Background:	Mitochondrial F0F1-ATP synthase is also called Complex V and it synthesis ATP from ADP and Pi using the proton motive force created by respiratory electron transport. ATP1 (ATMG01190/AT2G07698) is a subunit of mitochondrial F0F1-ATP synthase in Arabidopsis.
Synonyms:	ATP1, ATP SYNTHASE SUBUNIT 1
Immunogen:	KLH-conjugated synthetic peptide derived from N-terminal section of ATP1 protein in Arabidopsis thaliana ATMG01190, AT2G07698.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Serum Peptide affinity form antibody available upon request at info@phytoab.com.
Reconstitution:	Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	55 kDa (ATMG01190), 86 kDa (AT2G07698)
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa subsp. chinensis, Brassica napus, Brassica rapa subsp. oleifera, and 80-99% homologues with the sequence in Medicago truncatula, Sorghum bicolor, Solanum tuberosum, Gossypium raimondii, Solanum lycopersicum, Nicotiana tabacum, Triticum aestivum, Vitis vinifera, Glycine max, Zea mays subsp. mays, Cucumis sativus, Gossypium raimondii, Populus trichocarpa, Spinacia oleracea, Zea mays, Sorghum bicolor, Physcomitrella patens. For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

Lane 1: 15 μg mitochondria protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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