COX 6A / Anti-Cytochrome c oxidase subunit COX 6A, mitochondrial antibody

Catalog Number: PHY1126S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT4G37830

Product Information

Description:	Rabbit polyclonal antibody
Background:	Cytochrome c oxidase is the last enzyme in the respiratory electron transport chain of mitochondria and it is also called Complex IV. Cytochrome c oxidase receives an electron from each of four cytochrome c molecules, and transfers them to one oxygen molecule, converting molecular oxygen to two molecules of water. In higher plants mitochondria, Complex IV processes 14 subunits. COX 6A (AT4G37830) is one subunit of the Complex IV.
Synonyms:	COX 6A
Immunogen:	KLH-conjugated synthetic peptide derived from C-terminal section of COX 6A derived from Arabidopsis thaliana AT4G37830.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Serum Peptide affinity form antibody available upon request at info@phytoab.com.
Reconstitution:	Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	11 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Spinacia oleracea, Solanum lycopersicum, Oryza sativa Japonica Group, Brassica napus, Sorghum bicolor, Populus trichocarpa. For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

Lane 1: 10 μg mitochondria protein from Arabidopsis thaliana leaf.
Electrophoresis:Tricine-SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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