Dpt1 / Anti-Amino acid kinase family protein antibody

Catalog Number: PHY1029A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT3G18680

Product Information

Description:	Rabbit polyclonal antibody
Background:	Dpt1 (defect in psaA/B transcript accumulation1) is a novel photosystem (PS) I mutant in Arabidopsis. dpt1 mutants fail to grow photoautotrophically, and are impaired in the accumulation of psaA/B transcripts while the transcript levels for the remaining PSI subunits, for subunits of the PSII, the cyt-b6/f-complex, and the ribulose-1,5-bisphosphate carboxylase are comparable to the wild type.
Synonyms:	Dpt1
Immunogen:	KLH-conjugated synthetic peptide derived from C-terminal section of Dpt1 protein in Arabidopsis thaliana AT3G18680.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Immunogen affinity purified
Reconstitution:	Reconstitution with 150µl of 0.01 M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Applications:	Western Blot (1:500-1:1000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected Results:	36 / 30 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

Lane 1: 3 μg stromal protein from Arabidopsis thaliana leaf.
Lane 2: 6 μg stromal protein from Arabidopsis thaliana leaf.
Lane 3: 12 μg stromal protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:500 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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