RAM1 / Anti-Beta-amylase 5 antibody

Catalog Number: PHY2279A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT4G15210

Product Information

Description:	Rabbit polyclonal antibody
Background:	The Arabidopsis (Arabidopsis thaliana) genome contains nine β-amylase (BAM) genes, some of which play important roles in starch hydrolysis. RAM1(BAM5) has beta-amylase activity. It is major cytosolic beta-amylase isoform in rosette leaves and inflorescences stems.
Synonyms:	RAM1, ARABIDOPSIS THALIANA BETA-AMYLASE, AT-BETA-AMY, ATBETA-AMY, BAM5, BETA-AMYLASE 5, BMY1, REDUCED BETA AMYLASE 1
Immunogen:	KLH-conjugated synthetic peptide derived from central section of RAM1 protein in Arabidopsis thaliana AT4G15210.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Immunogen affinity purified
Reconstitution:	Reconstitution with 150µl of 0.01 M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	56 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica napus. For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

Lane 1: 10 μg soluble protein from Arabidopsis thaliana leaf.
Lane 2: 30 μg soluble protein from Arabidopsis thaliana leaf.
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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