LOGO-PHY

RAM1 / Anti-Beta-amylase 5 antibody

Catalog Number: PHY2279A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT4G15210

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

The Arabidopsis (Arabidopsis thaliana) genome contains nine β-amylase (BAM) genes, some of which play important roles in starch hydrolysis. RAM1(BAM5) has beta-amylase activity. It is major cytosolic beta-amylase isoform in rosette leaves and inflorescences stems.

  Synonyms:

RAM1, ARABIDOPSIS THALIANA BETA-AMYLASE, AT-BETA-AMY, ATBETA-AMY, BAM5, BETA-AMYLASE 5, BMY1, REDUCED BETA AMYLASE 1

  Immunogen:

KLH-conjugated synthetic peptide derived from central section of RAM1 protein in Arabidopsis thaliana AT4G15210.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01 M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

56 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica napus.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

 Application Example

Lane 1: 10 μg soluble protein from Arabidopsis thaliana leaf.
Lane 2: 30 μg soluble protein from Arabidopsis thaliana leaf.
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.