RPS6 / Anti-40S ribosomal protein S6-1 antibody

Catalog Number: PHY2025A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT4G31700

Product Information

Description:	Rabbit polyclonal antibody
Background:	RPS6A and RPS6B (AT5G10360) are fully redundant and essential during gametogenesis. It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Synonyms:	RPS6, ATRPS6, RIBOSOMAL PROTEIN S6, RPS6A
Immunogen:	KLH-conjugated synthetic peptide derived from N-terminal of RPS6 protein in Arabidopsis thaliana AT4G31700.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Immunogen affinity purified
Reconstitution:	Reconstitution with 150µl of 0.01 M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	28 / 30 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Glycine max, and 80-99% homologues with the sequence in Spinacia oleracea, Zea mays. The sequence of the synthetic peptide used for immunization is 94% homologues with the sequence in RPS6B (AT5G10360). For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

Lane 1: 12.5 μg cytosolic protein from Arabidopsis thaliana leaf.
Lane 2: 5 μg nuclear protein from Arabidopsis thaliana.
Lane 3: 20 μg total protein from Arabidopsis thaliana leaf.
Lane 4: 12 μg stromal protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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