线粒体TIM复合物亚基OEP16-3抗体

Catalog Number: PHY1471S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT2G42210

Product Information

Description:	Rabbit polyclonal antibody
Background:	The translocase of the inner membrane (TIM) is a complex of proteins found in the inner mitochondrial membrane of the mitochondria. OEP16-3 (AT2G42210) may be one subunit of the TIM complex.
Synonyms:	OEP16-3, ATOEP16-3
Immunogen:	KLH-conjugated synthetic peptide derived from N-terminal section of OEP16-3 protein in Arabidopsis thaliana AT2G42210.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Serum Peptide affinity form antibody available upon request at info@phytoab.com.
Reconstitution:	Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	17 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Solanum lycopersicum, Brassica napus, and 80-99% homologues with the sequence in Medicago truncatula, Oryza sativa Japonica Group, Sorghum bicolor, Spinacia oleracea, Hordeum vulgare, Populus trichocarpa, Vitis vinifera. For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

Lane 1: 10 μg mitochondria protein from Arabidopsis thaliana leaf.
Lane 2: 18 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis:15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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