CHS抗体

Catalog Number: PHY1168A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT5G13930

Product Information

Description:	Rabbit polyclonal antibody
Background:	Chalcone synthase (CHS) is a key enzyme involved in the biosynthesis of flavonoids. It is required for the accumulation of purple anthocyanins in leaves and stems. It is also involved in the regulation of auxin transport and the modulation of root gravitropism.
Synonyms:	CHS, ATCHS, CHALCONE SYNTHASE, TRANSPARENT TESTA 4, TT4
Immunogen:	KLH-conjugated synthetic peptide derived from C-terminal section of CHS protein in Arabidopsis thaliana AT5G13930.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Immunogen affinity purified
Reconstitution:	Reconstitution with 150µl of 0.01 M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	43 / 42 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	Among species analyzed, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Cucumis sativus. For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

20 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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