RPL4 / Anti-50S ribosomal protein L4, chloroplastic antibody

Catalog Number: PHY2500S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT1G07320

Product Information

Description:	Rabbit polyclonal antibody
Background:	50S ribosomal protein L4 (RPL4) binds directly to 23S ribosomal RNA.
Synonyms:	RPL4, EMB2784, EMBRYO DEFECTIVE 2784, PLASTID RIBOSOMAL PROTEIN L4, PRPL4, RIBOSOMAL PROTEIN L4
Immunogen:	KLH-conjugated synthetic peptide of RPL4 derived from Arabidopsis thaliana AT1G07320.
Form:	Lyophilized
Quantity:	150 µg
Puirification:	Serum Peptide affinity form antibody available upon request at info@phytoab.com.
Reconstitution:	Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	31 / 28 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	Among analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Vitis vinifera, Populus trichocarpa, Brassica napus, Brassica rapa. For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

Lane 1: 5 μg stromal protein from Arabidopsis thaliana leaf.
Lane 2: 10 μg stromal protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

首页 ꄲ 代理品牌 ꄲ RPL4 / Anti-50S ribosomal protein L4, chloroplastic antibody

搜索