Catalog Number: PHY0192A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT3G42050

Product Information


Rabbit polyclonal antibody


V-ATPases are multisubunit membrane protein complexes that use the energy provided by ATP hydrolysis to generate a proton gradient across various intracellular and plasma membranes. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow.


VHA-H ,V-ATPase, H


KLH-conjugated synthetic peptide of VHA-H derived from Arabidopsis thaliana AT3G42050.




150 µg


Immunogen affinity purified


Reconstitution with 150µl of 0.01M sterile PBS.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.


The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.


Application Information

  Recommended Dilution: 

Western Blot (1:500-1:1000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

50 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapaBrassica napus, and 80-99% homologues with the sequence in Medicago truncatulaGossypium raimondiiNicotiana tabacumCucumis sativusPopulus trichocarpa.
For more species homologues information, please contact tech support at tech@phytoab.com.


Application Example

20 µg total protein from Arabidopsis thaliana leaf.  
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.