液泡VHA-H抗体

Catalog Number: PHY0192A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT3G42050

Product Information

Description:	Rabbit polyclonal antibody
Background:	V-ATPases are multisubunit membrane protein complexes that use the energy provided by ATP hydrolysis to generate a proton gradient across various intracellular and plasma membranes. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow.
Synonyms:	VHA-H ,V-ATPase, H
Immunogen:	KLH-conjugated synthetic peptide of VHA-H derived from Arabidopsis thaliana AT3G42050.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Immunogen affinity purified
Reconstitution:	Reconstitution with 150µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:500-1:1000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	50 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa, Brassica napus, and 80-99% homologues with the sequence in Medicago truncatula, Gossypium raimondii, Nicotiana tabacum, Cucumis sativus, Populus trichocarpa. For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

20 µg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 12% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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