RIQ2抗体

Catalog Number: PHY0679S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT1G74730

Product Information

Description:	Rabbit polyclonal antibody
Background:	RIQ proteins contribute to NPQ and grana stacking in ways different from those of CURT1 functions, they may directly regulate the extent of grana stacking and, consequently, the optimization of the LHCII organization. RIQ proteins contain RIQ1 (AT5G08050) and RIQ2 (AT1G74730).
Synonyms:	RIQ2
Immunogen:	Recombinant protein of RIQ2 (45-198aa) derived from Arabidopsis thaliana AT1G74730.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Serum
Reconstitution:	Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	21 / 15 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

1-2 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 1.25 μg, and 2.5 μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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