SPY / Anti-SPINDLY antibody

Catalog Number: PHY1737A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT3G11540

Product Information

Description:	Rabbit polyclonal antibody
Background:	SPY is a N-acetyl glucosamine transferase that may glycosylate other molecules involved in GA signaling. SPY acts as both a repressor of GA responses and as a positive regulation of cytokinin signalling. It may be involved in reducing ROS accumulation in response to stress.
Synonyms:	SPY, SPINDLY
Immunogen:	Recombinant protein of SPY (834-914aa) derived from Arabidopsis thaliana AT3G11540.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Immunogen affinity purified
Reconstitution:	Reconstitution with 150µl of 0.01 M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Recommended Dilution:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected/apparent MW:	101 / 96 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

6 μg nuclear protein from Arabidopsis thaliana.
Electrophoresis: 10% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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