SNRK2.4/10 / Anti-Serine/threonine-protein kinase SRK2A/B antibody

Catalog Number: PHY0741A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT1G10940, AT1G60940

Product Information

Description:	Rabbit polyclonal antibody
Background:	SRK2A/B is a member of SNF1-related protein kinases (SnRK2) whose activity is activated by ionic (salt) and non-ionic (mannitol) osmotic stress.
Synonyms:	SNRK2.4/10, SNF1-RELATED PROTEIN KINASE 2.4/10, SNRK2-4/10, SRK2A/B, SUCROSE NONFERMENTING 1-RELATED PROTEIN KINASE 2-4/10
Immunogen:	KLH-conjugated synthetic peptide of SnRK2.4/10 derived from Arabidopsis thaliana AT1G10940, AT1G60940.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Immunogen affinity purified
Reconstitution:	Reconstitution with 150µl of 0.01 M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Applications:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected Results:	42 kDa (AT1G10940), 41 kDa (AT1G60940)
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	Among species analyzed, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa, Glycine max, Gossypium raimondii, Nicotiana tabacum, and 80-99% homologues with the sequence in Brassica napus, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera, Spinacia oleracea, Sorghum bicolor, Medicago truncatula, Triticum aestivum. The sequence of the synthetic peptide used for immunization is 81% homologues with the sequence in SnRK2.5 (AT5G63650). For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

Lane 1: 18 μg total protein from Arabidopsis thaliana leaf.
Lane 2: 10 μg cytosolic protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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