OLE2 / Anti-Oleosin 21.2 kDa antibody

Catalog Number: PHY0955A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT5G40420

Product Information

Description:	Rabbit polyclonal antibody
Background:	OLE2 is a protein found in oil bodies, and it is involved in seed lipid accumulation. Suppression of OLEO1(AT4G25140) (and OLEO2) resulted in an aberrant phenotype of embryo cells that contain unusually large oilbodies that are not normally observed in seeds.
Synonyms:	OLE2, OLEO2, OLEOSIN 2.
Immunogen:	KLH-conjugated synthetic peptide of OLE2 derived from Arabidopsis thaliana AT5G40420.
Form:	Lyophilized
Quantity:	150 µg
Purification:	Immunogen affinity purified
Reconstitution:	Reconstitution with 150µl of 0.01 M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
Shipping:	The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

Application Information

Applications:	Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Expected Results:	21 / 19 kDa
Confirmed Reactivity:	Arabidopsis thaliana
Predicted Reactivity:	Among analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica napus, Brassica rapa. For more species homologues information, please contact tech support at tech@phytoab.com.

Application Example

Lane 1: 20 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

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