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114162-64-0 X-gluc (CHX salt)

Catalog Number:G1281C
CAS Number:114162-64-0
G1281C-100mg
G1281C1G

PRODUCT DESCRIPTION

X-Gluc is a chromogenic substrate for beta-glucuronidase (GUS) which is encoded by the gusA (uid A) gene, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate. X-Gluc is often used in GUS staining of plant cells and tissue and in infections caused by E. coli.

Product Specifications:
(X-glu, X-glcA, BCIG)
5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronide cyclohexylammonium salt

MOLECULAR BIOLOGY GRADE

Formula: C14H13BrClNO7 • C6H13N

MW: 521.79 g/mol

Storage/Handling: Store desiccated at -20°C. Protect from light.
Soluble in DMF.

Purity: greater than 99% (HPLC)
Thin Layer Chromatogram: single spot
Biological Activity: indigo-blue assay
Elemental Analysis: Agrees with theoretical values

PubChem Chemical ID: 16760327

PRODUCT SPECIFICATIONS

Catalog ID G1281
CAS # 114162-64-0
MW 521.79 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.

TECHNICAL DOCUMENTATION

P Protocol
 
O Other
 
C Certificate of Analysis
 
S Safety Data Sheet
P
Histochemical Staining of GUS Reporter Activity

Pollen Staining for GUS Activity - adapted from a protocol by Dr. Daphne Preuss.

P
Gus Gene Assay in Transformed Plant Tissue

Procedures for GUS gene expression, fluorimetric and fluorigenic assays by Dr. Paul Bottino, Plant Molecular Genetics (retired), University of Maryland.

P
X-Gluc Stock Solution

X-Gluc Stock Solution - 20 mg/ml

C
COA - G1281

Certificate of Analysis - X-gluc

S
SDS (MSDS) - X-gluc

Safety Data Sheet

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