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NRPC3抗体

拟南芥有两个与酵母AC40同源的蛋白:At1G60620和At1G60850。At1G60620这种蛋白质以前被命名为AtRPAC43,因为它与酵母AC40同源,其预测分子量为43kDa。之后研究表明At1G60620主要被用于Pol III,与PolⅡ的Rpb3亚单位同源,因此命名为NRPC3。
针对NRPC3蛋白,我公司研发了相应的抗体PHY2053S。
Catalog Number: PHY2053S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT1G60620

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

NRPC3

  Synonyms:

NRPC3, ATRPAC43, RNA POLYMERASE I SUBUNIT 43, RPAC43

  Immunogen:

KLH-conjugated synthetic peptide of NRPC3 derived from Arabidopsis thaliana AT1G60620.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

43 / 45 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

15 μg total protein from Arabidopsis thaliana leaf.
Electrophoresis: 15% SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1 h.
Blocking: 5% skim milk at RT or 4℃ for 1 h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.