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AT5G11260 HY5 / Anti-Transcription factor HY5 antibody

Catalog Number: PHY0749S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT5G11260

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

HY5 is a basic leucine zipper (bZIP) transcription factor. Nuclear localization. It is involved in light-regulated transcriptional activation of G-box-containing promoters. Negatively regulated by Cop1. HY5 plays a role in anthocyanin accumulation in far-red light and blue light, but not in red light or in the dark.

  Synonyms:

HY5, ELONGATED HYPOCOTYL 5, REVERSAL OF THE DET PHENOTYPE 5, TED 5

  Immunogen:

KLH-conjugated synthetic peptide of HY5 derived from Arabidopsis thaliana AT5G11260.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water.
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &    
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Reference:

Xu Zheng, et al., The Plant Cell, Vol. 25: 115–133, January 2013.

 

Application Information

  Applications: 

Western Blot (1:1000-1:2000)
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

18 / 17 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa subsp. rapa, Brassica rapa subsp. pekinensis, Brassica rapa.
For more species homologues information, please contact tech support at tech@phytoab.com.