The epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cellsurface receptor for members of the epidermal growth factor family. Overexpression and/or hyperactivation of EGFR kinase is associated with several human cancers such as lung, glioblastoma, and epithelian tumors of the neck and head, leading to the development of anticancer therapeutics targeting EGFR. T790M, L858R and C797S mutations in EGFR cause resistance to known EGFR inhibitors. The EGFR(T790M/C797S/L858R) Kinase Assay Kit is designed to identify mutant-selective EGFR kinase inhibitors and to measure EGFR (T790M/C797S/L858R) kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
Materials Required But Not Provided:
Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
EGFR, Epidermal Growth Factor Receptor, ERBB, ErbB-1, HER1, MENA
The EGFR (T790M/C797S/L858R) Kinase Assay Kit comes in a convenient 96-well format, with enough purified recombinant EGFR(T790M/C797S/L858R) enzyme, EGFR substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Wang, S.,et al. J. Hematology & Oncology9:59 (2016)