PsbW is a nuclear-encoded PSII subunit and found in higher plants but not in cyanobacteria. PsbW has an apparent molecular mass of 6.1 kDa containing 54 amino acids. Loss of PsbW destabilizes the supramolecular organization of PSII.
PsbW, Photosystem II reaction center W protein, chloroplastic, PSII 6.1 kDa protein
KLH-conjugated synthetic peptide of PsbW derived fromArabidopsis thalianaAT2G30570.
Immunogen affinity purified
Reconstitution with 150µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:100-1:200) Note: Optimal dilutions/concentrations should be determined by the end user.
14 / 9 kDa
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence inBrassica rapa, Medicago truncatula. For more species homologues information, please contact tech support at firstname.lastname@example.org.
1-2 is thylakoid membrane protein from WT ofArabidopsis thalianaleaf containing 2 μg and 4 μg of chlorophyll, respectively. 3 is thylakoid membrane protein from Arabidopsis mutant with low accumulation of PSII containing 4 μg of chlorophyll. Electrophoresis:Tricine-SDS-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:200 dilution overnight at 4℃. Secondary antibody:1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.