PsbY subunit is encoded by the nucleus genome and is processed into two integral membrane proteins with identical topology (PsbY-1 and PsbY-2 with molecular masses of 4.673 kDa or 4.893 kDa, respectively) after imported into the chloroplast. PsbY has been suggested to bind manganese and is important for the redox control of cytochrome b559.
PsbY, PHOTOSYSTEM II BY, YCF32
KLH-conjugated synthetic peptide of PsbY derived fromArabidopsis thalianaAT1G67740.
Serum Peptide affinity form antibody available upon request at firstname.lastname@example.org.
Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
For more species homologues information, please contact tech support at email@example.com.
1-3 is thylakoid membrane protein from WT ofArabidopsis thalianaleaf containing 2 μg, 4 μg, and 5 μg of chlorophyll, respectively. 4 is thylakoid membrane protein from Arabidopsis mutant with low accumulation of PSII containing 5 μg of chlorophyll. Electrophoresis:Tricine-SDS-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:1000 dilution overnight at 4℃. Secondary antibody:1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.