HCF173 is localized in the chloroplast thylakoid membrane and is involved in the initiation of translation of the psbA mRNA. Mutants without HCF173 show high chlorophyll fluorescence phenotype and are severely affected in the accumulation of PSII subunits.
Recombinant, mature HCF173 protein inArabidopsis thalianaAT1G16720.
Protein A purified
Reconstitution with 150µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
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Lane 1: 10 μg total protein fromArabidopsis thalianaleaf. Lane 2: 50 μg total protein fromArabidopsis thalianaleaf. Lane 3: 100 μg total protein fromArabidopsis thalianaleaf. Electrophoresis:12.5% SDS-Urea-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:1000 dilution overnight at 4℃. Secondary antibody:1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.