ndhA, NAD(P)H dehydrogenase I subunit 1, NDH-1 subunit 1, NDH-A
KLH-conjugated synthetic peptide of ndhA protein derived fromSynechocystis sp. PCC 6803.
Serum Peptide affinity form antibody available upon request at email@example.com.
Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Synechocystis sp. PCC 6803
For more species homologues information, please contact tech support at firstname.lastname@example.org.
WT(12.5-100)is thylakoid membrane protein from WT ofSynechocystis sp. PCC 6803containing 0.625 μg, 1.25 μg, 2.5 μg, 3.75 μg and 5 μg of chlorophyll, respectively. A(100-12.5)is thylakoid membrane protein from mutant (ndhA) ofSynechocystis sp. PCC 6803containing 5 μg, 3.75 μg, 2.5 μg, 1.25 μg and 0.625 μg of chlorophyll, respectively. Electrophoresis:15% SDS-Urea-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 2h. Primary antibody:1:1000 dilution overnight at 4℃. Secondary antibody:1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.