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PsaE / Anti-PsaE subunit of photosystem I antibody PHY0162A

PsaE / Anti-PsaE subunit of photosystem I antibody 
Catalog Number: PHY0162A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana 
Database Access Number: AT4G28750

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

PsaE is part of the stromal side of photosystem I (PSI). In Arabidopsis thaliana, the two nuclear genes PsaE1 and PsaE2 code for PSI-E, and transcripts of PsaE1 are markedly more abundant than PsaE2 transcripts.

  Synonyms:

PsaE

  Immunogen:

KLH-conjugated synthetic peptide of PsaE derived from Arabidopsis thaliana AT4G28750.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:100-1:500) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

15 / 10 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Medicago truncatula, Zea mays.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Thylakoid membrane protein from Arabidopsis thaliana leaf containing 5 μg of chlorophyll. 
Electrophoresis: Tricine-SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:500 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.