PsaE is part of the stromal side of photosystem I (PSI). InArabidopsis thaliana, the two nuclear genesPsaE1andPsaE2code for PSI-E, and transcripts of PsaE1 are markedly more abundant than PsaE2 transcripts.
KLH-conjugated synthetic peptide of PsaE derived fromArabidopsis thalianaAT4G28750.
Immunogen affinity purified
Reconstitution with 150µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:100-1:500) Note: Optimal dilutions/concentrations should be determined by the end user.
15 / 10 kDa
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence inMedicago truncatula, Zea mays. For more species homologues information, please contact tech support at firstname.lastname@example.org.
Thylakoid membrane protein fromArabidopsis thalianaleaf containing 5 μg of chlorophyll. Electrophoresis:Tricine-SDS-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:500 dilution overnight at 4℃. Secondary antibody:1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.