网站首页ꄲ代理品牌ꄲPsaL / Anti-PsaL subunit of Photosystem I antibody PHY0122
PsaL / Anti-PsaL subunit of Photosystem I antibody PHY0122
PsaL / Anti-PsaL subunit of Photosystem I antibody Catalog Number: PHY0122 Size: 150 µg Confirmed reactivity: Arabidopsis thaliana Database Access Number: AT4G12800
Rabbit polyclonal antibody
PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin to ferredoxin. PsaL is binding pigments and has been shown to be involved in trimerization of PSI in cyanobacteria (but not in plants) and bind pigments in plants and cyanobacteria.
KLH-conjugated synthetic peptide of PsaL derived fromArabidopsis thalianaAT4G12800.
Protein A purified
Reconstitution with 150µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
18 / 15 kDa
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence inZea mays,and 80-99% homologues with the sequence inBrassica napus, Brassica rapa, Gossypium raimondii, Medicago truncatula, Populus trichocarpa, Solanum tuberosum, Solanum lycopersicum, Vitis vinifera, Cucumis sativus, Oryza sativa Japonica Group. For more species homologues information, please contact tech support at firstname.lastname@example.org.
1-3 is thylakoid membrane protein fromArabidopsis thalianaleaf containing 0.1 μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively. Electrophoresis:15% SDS-Urea-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:2000 dilution overnight at 4℃. Secondary antibody:1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.