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PsaN / Anti-PsaN subunit of photosystem I antibody PHY0123A

PsaN / Anti-PsaN subunit of photosystem I antibody
Catalog Number: PHY0123A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT5G64040

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Photosystem I (PSI) is a multisubunit complex that catalyses the final stage of the light reactions in chloroplasts. PsaN is the only subunit of photosystem I located entirely in the thylakoid lumen. It may be involved in the interaction between plastocyanin and the photosystem I complex.

  Synonyms:

PsaN, PSAN

  Immunogen:

KLH-conjugated synthetic peptide of PsaN derived from Arabidopsis thaliana AT5G64040.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

19 / 10 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Brassica napus, Gossypium raimondii, Vitis vinifera, Medicago truncatula, Cucumis sativus, Nicotiana tabacum, Oryza sativa Japonica Group, Zea mays, Solanum tuberosum, Chlamydomonas reinhardtii.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 1 μg, 2 μg, and 5 μg of chlorophyll, respectively. 
Electrophoresis: Tricine-SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.