Photosystem I (PSI) is a multisubunit complex that catalyses the final stage of the light reactions in chloroplasts. PsaN is the only subunit of photosystem I located entirely in the thylakoid lumen. It may be involved in the interaction between plastocyanin and the photosystem I complex.
KLH-conjugated synthetic peptide of PsaN derived fromArabidopsis thalianaAT5G64040.
Immunogen affinity purified
Reconstitution with 150µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
19 / 10 kDa
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence inBrassica napus, Gossypium raimondii, Vitis vinifera, Medicago truncatula, Cucumis sativus, Nicotiana tabacum, Oryza sativa Japonica Group, Zea mays, Solanum tuberosum, Chlamydomonas reinhardtii. For more species homologues information, please contact tech support at firstname.lastname@example.org.
1-3 is thylakoid membrane protein fromArabidopsis thalianaleaf containing 1 μg, 2 μg, and 5 μg of chlorophyll, respectively. Electrophoresis:Tricine-SDS-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:1000 dilution overnight at 4℃. Secondary antibody:1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.