PsaO has two transmembrane helices and a calculated mass of 10104 Da. PsaO is involved in the balancing of excitation energy between the two photosystems I (PSI) and II (PSII).
PsaO, Photosystem I subunit O, PSI-O, PHOTOSYSTEM I SUBUNIT O, PSAO
KLH-conjugated synthetic peptide of PsaO derived fromArabidopsis thalianaAT1G08380.
Serum Peptide affinity form antibody available upon request at email@example.com.
Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:5000) Note: Optimal dilutions/concentrations should be determined by the end user.
15 / 11 kDa
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence inMedicago truncatula. For more species homologues information, please contact tech support at firstname.lastname@example.org.
1-2 is thylakoid membrane protein from WT ofArabidopsis thalianaleaf containing 0.1 μg, 0.25 μg of chlorophyll, respectively. Electrophoresis:Tricine-SDS-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:5000 dilution overnight at 4℃. Secondary antibody:1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.