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YCF3 / Anti-YCF3 antibody PHY0440S

YCF3 / Anti-YCF3 antibody
Catalog Number: PHY0440S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: ATCG00360

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

YCF3 is essential for the assembly of the photosystem I (PSI) complex. In Chlamydomonas reinhardtii, it seems to act as a PSI specific chaperone facilitating the assembly of the complex by interacting with PsaA and PsaD.

  Synonyms:

YCF3

  Immunogen:

KLH-conjugated synthetic peptide of YCF3 derived from Arabidopsis thaliana ATCG00360.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

15 / 16 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Cucumis sativus, Gossypium raimondii, Populus trichocarpa, Solanum tuberosum, Nicotiana tabacum, Vitis vinifera, Oryza sativa Indica Group, Zea mays, Triticum aestivum, Hordeum vulgare subsp. Vulgare, Glycine max, Solanum lycopersicum, Spinacia oleracea, Physcomitrella patens.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

 Example 1

Lane 1: 10 ng recombinant protein of YCF3. 
Lane 2: 50 ng recombinant protein of YCF3. 
Lane 3: 100 ng recombinant protein of YCF3. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.