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YCF4 / Anti-Photosystem I assembly protein Ycf4 antibody PHY0441S

YCF4 / Anti-Photosystem I assembly protein Ycf4 antibody
Catalog Number: PHY0441S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: ATCG00520

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

YCF4 is a protein required for photosystem I assembly and stability.

  Synonyms:

YCF4.

  Immunogen:

KLH-conjugated synthetic peptide of YCF4 derived from Arabidopsis thaliana ATCG00520.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

21 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, and 80-99% homologues with the sequence in Oryza sativa Japonica Group, Vitis vinifera, Triticum aestivum, Zea mays, Setaria viridis, Panicum virgatum, Sorghum bicolor, Populus trichocarpa, Gossypium raimondii, Solanum tuberosum, Nicotiana tabacum, Spinacia oleracea.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.25 μg, 0.5 μg, and 1.25μg of chlorophyll, respectively. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.