YCF4 is a protein required for photosystem I assembly and stability.
KLH-conjugated synthetic peptide of YCF4 derived fromArabidopsis thalianaATCG00520.
Serum Peptide affinity form antibody available upon request at firstname.lastname@example.org.
Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence inBrassica napus,and 80-99% homologues with the sequence inSolanum tuberosum, Populus trichocarpa, Gossypium raimondii, Nicotiana tabacum, Oryza sativa Japonica Group, Zea mays, Setaria viridis, Panicum virgatum, Sorghum bicolor, Cucumis sativus, Vitis vinifera, Spinacia oleracea. For more species homologues information, please contact tech support at email@example.com.
1-3 is thylakoid membrane protein fromArabidopsis thalianaleaf containing 0.25 μg, 0.5 μg, and 1.25μg of chlorophyll, respectively. Electrophoresis:15% SDS-Urea-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:1000 dilution overnight at 4℃. Secondary antibody:1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.