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ATCG00540 PetA / Anti-Cytochrome f antibody

PetA / Anti-Cytochrome f antibody
Catalog Number: PHY0488A
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana 
Database Access Number: ATCG00540

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Cytochrome f is a component of cytochrome b6/f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.

  Synonyms:

PetA, photosynthetic electron transfer A, Cyt f.

  Immunogen:

KLH-conjugated synthetic peptide of PetA derived from Arabidopsis thaliana ATCG00540.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

35 / 34 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica napus, Oryza sativa Japonica Group, and 80-99% homologues with the sequence inNicotiana tabacum, Zea mays, Panicum virgatum, Sorghum bicolor, Glycine max, Vitis vinifera, Medicago truncatula f. tricycle, Gossypium raimondii, Medicago truncatula, Solanum tuberosum, Spinacia oleracea, Populus trichocarpa, Triticum aestivum, Hordeum vulgare subsp. Vulgare. 
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

 Example 1

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1 μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively.
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.