网站首页    代理品牌    AT2G36530 Enolase / Anti-Enolase antibody

AT2G36530 Enolase / Anti-Enolase antibody

Catalog Number: PHY0213
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT2G36530

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Enolases are key glycolytic enzymes that are highly conserved in prokaryotic and eukaryotic organisms and are among the most abundant cytosolic proteins.

  Synonyms:

Enolase, ENO2, ENOLASE 2, LOS2, LOW EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 2

  Immunogen:

KLH-conjugated synthetic peptide of Enolase derived from Arabidopsis thaliana AT2G36530.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150 µl of 0.01 M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

48 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Brassica rapa subsp. Oleifera, Solanum lycopersicum, Brassica napus, and 80-99% homologues with the sequence in Oryza sativa Japonica Group, Zea mays, Triticum aestivum, Spinacia oleracea.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 10 μg total protein from Arabidopsis thaliana leaf . 
Lane 2: 25 μg total protein from Arabidopsis thaliana leaf. 
Lane 3: 50 μg total protein from Arabidopsis thaliana leaf. 
Electrophoresis: 12.5% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.