ATP synthase subunit epsilon is a subunit of the CF0 subcomplex of chloroplast ATP synthase. This subunit may be involved in the regulation of the chloroplast ATP synthase activity.
AtpE, ATP synthase F1 sector epsilon subunit, F-ATPase epsilon subunit.
Recombinant, full length Epsilon subunit of chloroplast ATP synthase inArabidopsis thalianaATCG00470.
Protein A purified
Reconstitution with 150µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
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1-3 is thylakoid membrane protein fromArabidopsis thalianaleaf containing 0.5 μg, 1.25 μg, and 2 μg of chlorophyll, respectively. Electrophoresis:15% SDS-Urea-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:2000 dilution overnight at 4℃. Secondary antibody:1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.