Chloroplast ATP synthase consists of two structural domains, CF0 and CF1. CF1 contains the extramembraneous catalytic core and CF0 contains the membrane proton channel. Two domains are linked together by a central stalk and a peripheral stalk. ATP synthase subunit b is a subunit of the peripheral stalk.
AtpF, ATP synthase F(0) sector subunit b, CF0I
Recombinant, full length ATP synthase subunit b, chloroplastic Protein ofArabidopsis thalianaATCG00130.
Protein A purified
Reconstitution with 150µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
21 / 15 kDa
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1-3 is thylakoid membrane protein fromArabidopsis thalianaleaf containing 0.1 μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively. Electrophoresis:15% SDS-Urea-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:1000 dilution overnight at 4℃. Secondary antibody:1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.