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AtpF / Anti-CFoI subunits of chloroplast ATP synthase antibody PHY0316

AtpF / Anti-CFoI subunits of chloroplast ATP synthase antibody
Catalog Number: PHY0316
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: ATCG00130

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Chloroplast ATP synthase consists of two structural domains, CF0 and CF1. CF1 contains the extramembraneous catalytic core and CF0 contains the membrane proton channel. Two domains are linked together by a central stalk and a peripheral stalk. ATP synthase subunit b is a subunit of the peripheral stalk.

  Synonyms:

AtpF, ATP synthase F(0) sector subunit b, CF0I

  Immunogen:

Recombinant, full length ATP synthase subunit b, chloroplastic Protein of Arabidopsis thaliana ATCG00130.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

21 / 15 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1 μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.