ATPI is a subunit of ATPase complex CF0, which is a proton channel that supplies the proton motive force to drive ATP synthesis by CF1 portion of the complex.
ATPI, ATP synthase F0 sector subunit a, F-ATPase subunit IV
KLH-conjugated synthetic peptide of ATPI derived fromArabidopsis thalianaATCG00150.
Immunogen affinity purified Peptide affinity form antibody available upon request at firstname.lastname@example.org.
Reconstitution with 150µl of 0.01M sterile PBS. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
27 / 17 kDa
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence inTriticum aestivum, Sorghum bicolor. For more species homologues information, please contact tech support at email@example.com.
1-3 is thylakoid membrane protein from WT ofArabidopsis thalianaleaf containing 1 μg, 2 μg, and 4 μg of chlorophyll, respectively. 4 is thylakoid membrane protein from Arabidopsis mutant with low accumulation of ATPase containing 4 μg of chlorophyll. Electrophoresis:Tricine-SDS-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:1000 dilution overnight at 4℃. Secondary antibody:1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.