ATPI / Anti-CFoIV subunits of chloroplast ATP synthase antibody PHY1355A

  Description:

Rabbit polyclonal antibody

  Background:

ATPI is a subunit of ATPase complex CF0, which is a proton channel that supplies the proton motive force to drive ATP synthesis by CF1 portion of the complex.

  Synonyms:

ATPI, ATP synthase F0 sector subunit a, F-ATPase subunit IV

  Immunogen:

KLH-conjugated synthetic peptide of ATPI derived from Arabidopsis thaliana ATCG00150.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Immunogen affinity purified
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

27 / 17 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Triticum aestivum, Sorghum bicolor.
For more species homologues information, please contact tech support at tech@phytoab.com.

1-3 is thylakoid membrane protein from WT of Arabidopsis thaliana leaf containing 1 μg, 2 μg, and 4 μg of chlorophyll, respectively. 
4 is thylakoid membrane protein from Arabidopsis mutant with low accumulation of ATPase containing 4 μg of chlorophyll. 
Electrophoresis: Tricine-SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:10000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.