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CPN60B1/2 / Anti-Chaperonin 60 subunit beta 1/2, chloroplastic antibody AT1G55490, AT3G13470

Catalog Number: PHY0372S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT1G55490, AT3G13470

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Type I chaperonins are large, double ring complexes invovled in mediating the folding of unfolded proteins. In chloroplasts, chaperonin complex is designated Cpn60 complex. In Arabidopsis, two Cpn60α subunits and four Cpn60β subunits are present.Cpn60β1 (AT1G55490) and Cpn60β2 (AT3G13470) is the main Cpn60β subunit in chloroplasts. This antibody recognizes both proteins.

  Synonyms:

CPN60B1/2

  Immunogen:

KLH-conjugated synthetic peptide of CPN60B1 derived from Arabidopsis thaliana AT1G55490 and AT3G13470.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:5000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

64 / 58 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Solanum tuberosum, Chlamydomonas reinhardtii.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 2.75 μg stromal protein from Arabidopsis thaliana leaf. 
Lane 2: 6 μg stromal protein from Arabidopsis thaliana leaf. 
Lane 3: 12 μg stromal protein from Arabidopsis thaliana leaf. 
Electrophoresis: 12.5% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:5000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.