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CPN10-I / Anti-Chaperonin 10 subunit, mitochondrial antibody AT1G14980

Catalog Number: PHY0375S
Size: 150 µg
Confirmed reactivity: Arabidopsis thaliana
Database Access Number: AT1G14980

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

Co-chaperonin complex is required for substrate encapsulation during assisting the folding of the unfolded protein with the chaperonin complex. Co-chaperonin also termed as GroES, Cpn10 and Hsp10. CPN10-I (AT1G14980) is a mitochondrial-localized chaperonin 10 protein and its mRNA is upregulated in response to heat shock treatment and is expressed uniformly in various organs.

  Synonyms:

CPN10-I, CHAPERONIN 10

  Immunogen:

KLH-conjugated synthetic peptide of CPN10-I derived from Arabidopsis thaliana AT1G14980.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

11 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence in Populus trichocarpa.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

Lane 1: 11.4 μg stromal protein from Arabidopsis thaliana leaf. 
Lane 2: 17.1 μg stromal protein from Arabidopsis thaliana leaf. 
Electrophoresis: Tricine-SDS-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.