Co-chaperonin complex is required for substrate encapsulation during assisting the folding of the unfolded protein with the chaperonin complex. Co-chaperonin also termed as GroES, Cpn10 and Hsp10. CPN10-I (AT1G14980) is a mitochondrial-localized chaperonin 10 protein and its mRNA is upregulated in response to heat shock treatment and is expressed uniformly in various organs.
CPN10-I, CHAPERONIN 10
KLH-conjugated synthetic peptide of CPN10-I derived fromArabidopsis thalianaAT1G14980.
Serum Peptide affinity form antibody available upon request at email@example.com.
Reconstitution with 150µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution.
1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 80-99% homologues with the sequence inPopulus trichocarpa. For more species homologues information, please contact tech support at firstname.lastname@example.org.
Lane 1: 11.4 μg stromal protein fromArabidopsis thalianaleaf. Lane 2: 17.1 μg stromal protein fromArabidopsis thalianaleaf. Electrophoresis:Tricine-SDS-PAGE Transfer:blotting to NC (nitrocellulose) membrane for 1h. Blocking:5% skim milk at RT or 4℃ for 1h. Primary antibody:1:2000 dilution overnight at 4℃. Secondary antibody:1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.