Phenylethynylpyrene (PEP) fluorophore is a polyaromatic hydrocarbon label with high sensitivity to microenvironment. Similarly to pyrene, PEP dye readily forms excimers. However, PEP fluorescence is more red-shifted
PEP can be used as microenvironment probe, and as a label for assays based on excimer formation.
This reagent contains triethyleneglycol linker to facilitate dissolution of non-polar PEP dye in organo-aqueous labeling reaction mixtures. With this azide, and Click Chemistry, it is easy to turn any molecule bearing alkyne into PEP-labeled probe.
Spectra of PEP (phenylethynylpyrene) in ethanol
Customers also purchased with this product
Water soluble sulfo-Cyanine5 fluorescent dye azide for Click Chemistry.
Reagent for fluorescent labeling with pyrene polycyclic aromatic hydrocarbon via Click Chemistry.
Coumarin 343 is a blue emitting fluorophore that can serve as a FRET donor for fluorescein (FAM). This azide derivative can be conjugated with alkynes by Click chemistry. An extended linker arm between the fluorophore and the azide group enhances solubility, and provides spatial separation between the dye and the biomolecule.
||off white / yellowish solid
||soluble in dichloromethane, chloroform, moderately soluble in DMSO, DMF, acetonitrile
||NMR 1H (95%)
||Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.